Clinical and paraclinical changes in experimental colisepticemia in neonatal calves

Mohammad Reza Mokhber Dezfoulie1, Samad Lotfollahzadeh1*, Masoomeh Heidari2, Gholamreza Nikbakht Boroujeni3

1.Department of Internal Medicine, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
2.Iran Veterinary Organization (IVO), Tehran, Iran .
3. Department of Microbiology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran

Samad Lotfollahzadeh ORCID: 0000-0002-6549-7922


 *Samad Lotfollahzadeh: samadlzadeh@ut.ac.ir

Received:
24.05.2019,  Accepted: 17.07.2019,  Available online: 06.08.2019

ABSTRACT


The present study aim was to assess changes in clinical signs and plasma cytokines in calves experimentally infected with Escherchia coli and use them as a tool to diagnose colisepticemia. Ten healthy Holstein-Frisian bull calves were used for this experimental study. Experimental colisepticemia was induced in calves with intravenous injection of 1.5 × 109 CFU of O111:H8 strain of E.coli. Clinical scores were recorded before induction of septicemia, every 30 minutes for 8 hours, every hour from 8 till 12 h, every 3 hours from  12  till  24  hours  after  bacterial  challenge.  Blood  samples  were  collected  to determine  plasma  concentration  of  tumor  necrosis  factor-α  (TNF-α) and  gamma interferon  gamma  (IFN-γ) before  and  after  E.coli  IV  injection.  Blood  culture  was performed before and after bacterial challenge to confirm colisepticemia. The present study showed that total clinical score of the calves increased with a simultaneous significant rise in plasma concentration of TNF-α and INF-γ during septicemia period (P< 0.05). Changes in the heart and respiratory rate during septicemia and using clinical scoring are not enough to assess the magnitude of infection and disease progress; therefore, it is recommended some laboratory tests be used for better evaluation of clinical status of the septic calves

Keywords: experimental colisepticemia, calf, clinical scoring, tumor necrosis factor-α, interferon gamma